제품 > 항체 > 단일클론항체(mAb)

VCAM1 Rabbit PolymAb® (A19131PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VCAM1 Rabbit PolymAb® (A19131PM)

Western blot analysis of various lysates using VCAM1 Rabbit PolymAb® (A19131PM) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): RAW 264.7
Exposure time: 10s.

ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

Confocal imaging of paraffin-embedded Human kidney tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

Confocal imaging of paraffin-embedded Human spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

Confocal imaging of paraffin-embedded Mouse spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameVCAM1 Rabbit PolymAb®
Catalog No.A19131PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 640-739 of human VCAM1 (P19320).
SequenceETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV
Gene ID
Swiss Prot
SynonymsCD106; INCAM-100
Calculated MW38kDa/71KDa/74KDa/81KDa
Observed MW110kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2000 - 1:12000
  • IHC-P 1:1000 - 1:4000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, C2C12, Mouse spleen, Rat spleen
Cellular locationMembrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - VCAM1 Rabbit PolymAb® (A19131PM)}

    Western blot - VCAM1 Rabbit PolymAb® (A19131PM)

    Western blot analysis of various lysates using VCAM1 Rabbit PolymAb® (A19131PM) at 1:2000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): RAW 264.7
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunohistochemistry - VCAM1 Rabbit PolymAb® (A19131PM)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

    Confocal imaging of paraffin-embedded Human kidney tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

    Confocal imaging of paraffin-embedded Human spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)}

    Immunofluorescence - VCAM1 Rabbit PolymAb® (A19131PM)

    Confocal imaging of paraffin-embedded Mouse spleen tissue using VCAM1 Rabbit PolymAb® (A19131PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)