Western blot - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Western blot analysis of various lysates using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at 1:9000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Confocal imaging of paraffin-embedded Rat testis tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Confocal imaging of paraffin-embedded Mouse testis tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunofluorescence - VDAC 1/2/3 Rabbit PolymAb® (A27273PM)
Confocal imaging of paraffin-embedded Human tonsil tissue using VDAC 1/2/3 Rabbit PolymAb® (A27273PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.