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VDAC2 Rabbit pAb (A18683)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VDAC2 Rabbit pAb (A18683)

Western blot analysis of various lysates using VDAC2 Rabbit pAb (A18683) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Human brain using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Rat heart using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - VDAC2 Rabbit pAb (A18683)

Immunofluorescence analysis of NIH/3T3 cells using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDAC2 Rabbit pAb (A18683)

Immunofluorescence analysis of PC-12 cells using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVDAC2 Rabbit pAb
Catalog No.A18683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the voltage-dependent anion channel pore-forming family of proteins that are considered the main pathway for metabolite diffusion across the mitochondrial outer membrane. The encoded protein is also thought to be involved in the mitochondrial apoptotic pathway via regulation of BCL2-antagonist/killer 1 protein activity. Pseudogenes have been identified on chromosomes 1, 2, 12 and 21, and alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human VDAC2 (NP_003366.2).
SequenceMATHGQTCARPMCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIED
Gene ID
Swiss Prot
SynonymsPOR; VDAC2
Calculated MW32kDa
Observed MW35kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, HeLa, U-251MG, Mouse testis, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular locationMitochondrion outer membrane.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - VDAC2 Rabbit pAb (A18683)}

Western blot - VDAC2 Rabbit pAb (A18683)

Western blot analysis of various lysates using VDAC2 Rabbit pAb (A18683) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)}

Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Human brain using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)}

Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)}

Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDAC2 Rabbit pAb (A18683)}

Immunohistochemistry - VDAC2 Rabbit pAb (A18683)

Immunohistochemistry analysis of paraffin-embedded Rat heart using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - VDAC2 Rabbit pAb (A18683)}

Immunofluorescence - VDAC2 Rabbit pAb (A18683)

Immunofluorescence analysis of NIH/3T3 cells using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDAC2 Rabbit pAb (A18683)}

Immunofluorescence - VDAC2 Rabbit pAb (A18683)

Immunofluorescence analysis of PC-12 cells using VDAC2 Rabbit pAb (A18683) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A18683? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)