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VDR Rabbit mAb (A23289)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VDR Rabbit mAb (A23289)

Western blot analysis of various lysates, using VDR Rabbit mAb (A23289) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - VDR Rabbit mAb (A23289)

Immunofluorescence analysis of paraffin-embedded rat kidney using VDR Rabbit mAb (A23289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDR Rabbit mAb (A23289)

Immunofluorescence analysis of T-47D cells using VDR Rabbit mAb (A23289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - VDR Rabbit mAb (A23289)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg VDR Rabbit mAb (A23289). Western blot was performed from the immunoprecipitate using VDR Rabbit mAb (A23289) at a dilition of 1:1000.

Overview

Product nameVDR Rabbit mAb
Catalog No.A23289
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60266
This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human VDR(NP_000367.1).
SequenceMEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEV
Gene ID
Swiss Prot
SynonymsNR1I1; PPP1R163; VDR
Calculated MW48kDa
Observed MW48kDa/54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:500 - 1:2000
  • IF/ICC 1:100 - 1:400
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
  • ChIP 5μg antibody for 5μg-10μg of Chromatin
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesT-47D, HeLa, NIH/3T3, Jurkat
Cellular locationNucleus, Cytoplasm, Cytosol.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - VDR Rabbit mAb (A23289)}

Western blot - VDR Rabbit mAb (A23289)

Western blot analysis of various lysates, using VDR Rabbit mAb (A23289) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit mAb (A23289)}

Immunohistochemistry - VDR Rabbit mAb (A23289)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using VDR Rabbit mAb (A23289) at a dilution of 1:500 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - VDR Rabbit mAb (A23289)}

Immunofluorescence - VDR Rabbit mAb (A23289)

Immunofluorescence analysis of paraffin-embedded rat kidney using VDR Rabbit mAb (A23289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDR Rabbit mAb (A23289)}

Immunofluorescence - VDR Rabbit mAb (A23289)

Immunofluorescence analysis of T-47D cells using VDR Rabbit mAb (A23289) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - VDR Rabbit mAb (A23289)}

Immunoprecipitation - VDR Rabbit mAb (A23289)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg VDR Rabbit mAb (A23289). Western blot was performed from the immunoprecipitate using VDR Rabbit mAb (A23289) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)