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VDR Rabbit pAb (A11743)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - VDR Rabbit pAb (A11743)

Western blot analysis of various lysates using VDR Rabbit pAb (A11743) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Rat liver using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of PC-12 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of NIH-3T3 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of HeLa cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameVDR Rabbit pAb
Catalog No.A11743
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human VDR (NP_000367.1).
SequenceMEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDV
Gene ID
Swiss Prot
SynonymsNR1I1; PPP1R163; VDR
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HT-29
Cellular locationNucleus.

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ABclonal:Western blot - VDR Rabbit pAb (A11743)}

Western blot - VDR Rabbit pAb (A11743)

Western blot analysis of various lysates using VDR Rabbit pAb (A11743) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)}

Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)}

Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - VDR Rabbit pAb (A11743)}

Immunohistochemistry - VDR Rabbit pAb (A11743)

Immunohistochemistry analysis of paraffin-embedded Rat liver using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of PC-12 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of NIH-3T3 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of HeLa cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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