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WISP2 Rabbit pAb (A7456)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WISP2 Rabbit pAb (A7456)

Western blot analysis of lysates from Mouse ovary, using WISP2 Rabbit pAb (A7456) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - WISP2 Rabbit pAb (A7456)

Immunohistochemistry analysis of paraffin-embedded Rat testis using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - WISP2 Rabbit pAb (A7456)

Immunohistochemistry analysis of paraffin-embedded Human colon using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - WISP2 Rabbit pAb (A7456)

Immunofluorescence analysis of HeLa cells using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - WISP2 Rabbit pAb (A7456)

Immunofluorescence analysis of MCF7 cells using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameWISP2 Rabbit pAb
Catalog No.A7456
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 164-250 of human WISP2 (NP_003872.1).
SequenceGQGGGLGTQPLPAQGPQFSGLVSSLPPGVPCPEWSTAWGPCSTTCGLGMATRVSNQNRFCRLETQRRLCLSRPCPPSRGRSPQNSAF
Gene ID
Swiss Prot
SynonymsCT58; WISP2; CTGF-L
Calculated MW27kDa
Observed MW27KD
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse ovary
Cellular locationSecreted
Customer validation

WB(Homo sapiens, Rattus norvegicus)

Co-IP(Homo sapiens)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - WISP2 Rabbit pAb (A7456)}

Western blot - WISP2 Rabbit pAb (A7456)

Western blot analysis of lysates from Mouse ovary, using WISP2 Rabbit pAb (A7456) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - WISP2 Rabbit pAb (A7456)}

Immunohistochemistry - WISP2 Rabbit pAb (A7456)

Immunohistochemistry analysis of paraffin-embedded Rat testis using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - WISP2 Rabbit pAb (A7456)}

Immunohistochemistry - WISP2 Rabbit pAb (A7456)

Immunohistochemistry analysis of paraffin-embedded Human colon using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - WISP2 Rabbit pAb (A7456)}

Immunofluorescence - WISP2 Rabbit pAb (A7456)

Immunofluorescence analysis of HeLa cells using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - WISP2 Rabbit pAb (A7456)}

Immunofluorescence - WISP2 Rabbit pAb (A7456)

Immunofluorescence analysis of MCF7 cells using WISP2 Rabbit pAb (A7456) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7456? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)