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WNT5B Rabbit pAb (A8313)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WNT5B Rabbit pAb (A8313)

Western blot analysis of lysates from Mouse ovary, using WNT5B Rabbit pAb (A8313) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameWNT5B Rabbit pAb
Catalog No.A8313
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 94% and 80% amino acid identity to the mouse Wnt5b protein and the human WNT5A protein, respectively. Alternative splicing of this gene generates 2 transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human WNT5B (NP_110402.2).
SequenceASVFGRVMQIGSRETAFTHAVSAAGVVNAISRACREGELSTCGCSRTARPKDLPRDWLWGGCGDNVEYGYRFAKEFVDAREREKNFAKGSEEQGRVLMNLQNNEAGRRAVYKMADVACKCHGVSGSCSLKTCWLQLAEFRKVGDRLKEKYDSAAAMRVTRKGRLELVNSRFTQPTPEDLVYVDPSPDYCLRNESTGSLGT
Gene ID
Swiss Prot
SynonymsWNT5B
Calculated MW40kDa
Observed MW45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse ovary
Cellular locationSecreted, extracellular matrix, extracellular space.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - WNT5B Rabbit pAb (A8313)}

Western blot - WNT5B Rabbit pAb (A8313)

Western blot analysis of lysates from Mouse ovary, using WNT5B Rabbit pAb (A8313) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)}

Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Rat bone marrow using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)}

Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - WNT5B Rabbit pAb (A8313)}

Immunohistochemistry - WNT5B Rabbit pAb (A8313)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using WNT5B Rabbit pAb (A8313) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)