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WT1 Rabbit mAb (A2446)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of various lysates using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of lysates from Rat testis using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Overview

Product nameWT1 Rabbit mAb
Catalog No.A2446
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2610
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544).
SequencePPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGV
Gene ID
Swiss Prot
SynonymsGUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; WT1
Calculated MW49kDa
Observed MW51-60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesOVCAR3, K-562, Mouse testis, Rat testis
Cellular locationCytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm.
Customer validation

IF(Mus musculus)

IHC(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - WT1 Rabbit mAb (A2446)}

Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of various lysates using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - WT1 Rabbit mAb (A2446)}

Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of lysates from Rat testis using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - WT1 Rabbit mAb (A2446)}

Western blot - WT1 Rabbit mAb (A2446)

Western blot analysis of lysates from Mouse testis using WT1 Rabbit mAb (A2446) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)}

Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human Ovarian Serous Carcinoma tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)}

Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - WT1 Rabbit mAb (A2446)}

Immunohistochemistry - WT1 Rabbit mAb (A2446)

Immunohistochemistry analysis of paraffin-embedded Human testis tissue using WT1 Rabbit mAb (A2446) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A2446? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)