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WT1 Rabbit pAb (A16298)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - WT1 Rabbit pAb (A16298)

Western blot analysis of various lysates using WT1 Rabbit pAb (A16298) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)

Confocal immunofluorescence analysis of Hela cells using WT1 Rabbit pAb (A16298) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)

Confocal immunofluorescence analysis of U-2 OS cells using WT1 Rabbit pAb (A16298) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)

Immunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit pAb (A16298) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameWT1 Rabbit pAb
Catalog No.A16298
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human WT1 (NP_001185480.1).
SequenceMEKGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHGVFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKPYQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFSCRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL
Gene ID
Swiss Prot
SynonymsGUD; AWT1; WAGR; WT-1; WT33; NPHS4; WIT-2; WT1
Calculated MW49kDa
Observed MW49-60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse uterus, Rat kidney, Rat testis
Cellular locationCytoplasm, Nucleus, Nucleus speckle, nucleolus, nucleoplasm.

Documents

Certificate of Compliance

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ABclonal:Western blot - WT1 Rabbit pAb (A16298)}

Western blot - WT1 Rabbit pAb (A16298)

Western blot analysis of various lysates using WT1 Rabbit pAb (A16298) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)}

Immunofluorescence - WT1 Rabbit pAb (A16298)

Confocal immunofluorescence analysis of Hela cells using WT1 Rabbit pAb (A16298) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)}

Immunofluorescence - WT1 Rabbit pAb (A16298)

Confocal immunofluorescence analysis of U-2 OS cells using WT1 Rabbit pAb (A16298) at dilution of 1:200. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - WT1 Rabbit pAb (A16298)}

Immunofluorescence - WT1 Rabbit pAb (A16298)

Immunofluorescence analysis of OVCAR-3 cells using WT1 Rabbit pAb (A16298) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)