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XBP1 Rabbit pAb (A1731)

Review (1)Publications (28) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - XBP1 Rabbit pAb (A1731)

Western blot analysis of lysates from Mouse spleen, using XBP1 Rabbit pAb (A1731) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - XBP1 Rabbit pAb (A1731)

Immunofluorescence analysis of NIH/3T3 cells using XBP1 Rabbit pAb (A1731) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for XBP1 Rabbit pAb(A1731)

Overview

Product nameXBP1 Rabbit pAb
Catalog No.A1731
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 277-376 of human XBP1 (NP_005071.2).
SequenceNVVVKIEEAPLSPSENDHPEFIVSVKEEPVEDDLVPELGISNLLSSSHCPKPSSCLLDAYSDCGYGGSLSPFSDMSSLLGVNHSWEDTFANELFPQLISV
Gene ID
Swiss Prot
SynonymsXBP2; TREB5; XBP-1; TREB-5; XBP1
Calculated MW29kDa
Observed MW60kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse spleen
Cellular locationCytoplasm, Endoplasmic reticulum membrane, Endoplasmic reticulum, Membrane, Nucleus, Peripheral membrane protein, Single-pass type II membrane protein.
Customer validation

(RAW264.7)

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Bos taurus, Gallus gallus, Sus scrofa, Anatinae, Canis lupus familiaris)

IHC(Rattus norvegicus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - XBP1 Rabbit pAb (A1731)}

Western blot - XBP1 Rabbit pAb (A1731)

Western blot analysis of lysates from Mouse spleen, using XBP1 Rabbit pAb (A1731) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - XBP1 Rabbit pAb (A1731)}

Immunofluorescence - XBP1 Rabbit pAb (A1731)

Immunofluorescence analysis of NIH/3T3 cells using XBP1 Rabbit pAb (A1731) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)