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XBP1S Rabbit pAb (A22546)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - XBP1S Rabbit pAb (A22546)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with XBP1S, using XBP1S Rabbit pAb (A22546) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - XBP1S Rabbit pAb (A22546)

Western blot analysis of lysates from Rat lung, using XBP1S Rabbit pAb (A22546) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of Hep-G2 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of HT-29 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of NIH/3T3 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of Hep-G2 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of HT-29 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameXBP1S Rabbit pAb
Catalog No.A22546
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 188-287 of human XBP1S. (NP_001073007.1).
SequenceSDSESDILLGILDNLDPVMFFKCPSPEPASLEELPEVYPEGPSSLPASLSLSVGTSSAKLEAINELIRFDHIYTKPLVLEIPSETESQANVVVKIEEAPL
Gene ID
Swiss Prot
SynonymsXBP2; TREB5; XBP-1; TREB-5; XBP1S
Calculated MW40KDa
Observed MW60kDa/55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T transfected with XBP1S, Rat lung
Cellular location
Customer validation

WB(Rattus norvegicus)

RT-qPCR(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - XBP1S Rabbit pAb (A22546)}

Western blot - XBP1S Rabbit pAb (A22546)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with XBP1S, using XBP1S Rabbit pAb (A22546) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - XBP1S Rabbit pAb (A22546)}

Western blot - XBP1S Rabbit pAb (A22546)

Western blot analysis of lysates from Rat lung, using XBP1S Rabbit pAb (A22546) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)}

Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of Hep-G2 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)}

Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of HT-29 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)}

Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of NIH/3T3 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)}

Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of Hep-G2 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XBP1S Rabbit pAb (A22546)}

Immunofluorescence - XBP1S Rabbit pAb (A22546)

Immunofluorescence analysis of HT-29 cells using XBP1S Rabbit pAb (A22546) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)