제품 > 항체 > 다중클론항체(pAb)

XK Rabbit pAb (A15109)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - XK Rabbit pAb (A15109)

Western blot analysis of various lysates using XK Rabbit pAb (A15109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of C6 cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of L929 cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of U-2 OS cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameXK Rabbit pAb
Catalog No.A15109
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This locus controls the synthesis of the Kell blood group 'precursor substance' (Kx). Mutations in this gene have been associated with McLeod syndrome, an X-linked, recessive disorder characterized by abnormalities in the neuromuscular and hematopoietic systems. The encoded protein has structural characteristics of prokaryotic and eukaryotic membrane transport proteins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 59-138 of human XK (NP_066569.1).
SequenceHRDLSRDRPLVLLLHLLQLGPLFRCFEVFCIYFQSGNNEEPYVSITKKRQMPKNGLSEEIEKEVGQAEGKLITHRSAFSR
Gene ID
Swiss Prot
SynonymsKX; NA; NAC; X1k; XKR1; XK
Calculated MW51kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesmouse pancreas, rat pancreas
Cellular locationMembrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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ABclonal:Western blot - XK Rabbit pAb (A15109)}

Western blot - XK Rabbit pAb (A15109)

Western blot analysis of various lysates using XK Rabbit pAb (A15109) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)}

Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of C6 cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)}

Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of L929 cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - XK Rabbit pAb (A15109)}

Immunofluorescence - XK Rabbit pAb (A15109)

Immunofluorescence analysis of U-2 OS cells using XK Rabbit pAb (A15109) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)