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Yipf6 Rabbit pAb (A22875)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Western blot - Yipf6 Rabbit pAb (A22875)

Western blot analysis of lysates from Rat brain using Yipf6 Rabbit pAb (A22875) at 1:400 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Enhanced Kit (RM00021).
Exposuretime:90s.

Overview

Product nameYipf6 Rabbit pAb
Catalog No.A22875
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity. Acts upstream of or within intestinal epithelial cell development. Located in COPII-coated ER to Golgi transport vesicle; cis-Golgi network; and trans-Golgi network. Orthologous to human YIPF6 (Yip1 domain family member 6).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-83 of mouse Yipf6(NP_997516.1).
SequenceMAEAEDSPGEQEAAASKPLFAGLSDVSISQDIPIEGEITIPSRARAQEHDSSTLNESIRRTIMRDLKAVGRKFMHVLYPRKSN
Gene ID
Swiss Prot
SynonymsA430107J06Rik; Yipf6
Calculated MW26kDa
Observed MW26kDa
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesRat brain
Cellular locationGolgi apparatus, Endoplasmic reticulum, Vesicles

Documents

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    ABclonal:Western blot - Yipf6 Rabbit pAb (A22875)}

    Western blot - Yipf6 Rabbit pAb (A22875)

    Western blot analysis of lysates from Rat brain using Yipf6 Rabbit pAb (A22875) at 1:400 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Enhanced Kit (RM00021).
    Exposuretime:90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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