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ZNF207 Rabbit pAb (A20177)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF207 Rabbit pAb (A20177)

Western blot analysis of various lysates using ZNF207 Rabbit pAb (A20177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded rat brain using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded human esophageal using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameZNF207 Rabbit pAb
Catalog No.A20177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables microtubule binding activity. Involved in several processes, including mitotic nuclear division; mitotic spindle assembly checkpoint signaling; and protein stabilization. Located in kinetochore; nuclear lumen; and spindle matrix.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human ZNF207 (NP_003448.1).
SequenceMGRKKKKQLKPWCWYCNRDFDDEKILIQHQKAKHFKCHICHKKLYTGPGLAIHCMQVHKETIDAVPNAIPGRTDIELEIYGMEGI
Gene ID
Swiss Prot
SynonymsBuGZ; hBuGZ; ZNF207
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat brain
Cellular locationcytoplasm, nucleolus, nucleoplasm, nucleus, spindle, spindle matrix
Customer validation

IF(Homo sapiens)

Documents

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ABclonal:Western blot - ZNF207 Rabbit pAb (A20177)}

Western blot - ZNF207 Rabbit pAb (A20177)

Western blot analysis of various lysates using ZNF207 Rabbit pAb (A20177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)}

Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded rat brain using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)}

Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded human esophageal using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF207 Rabbit pAb (A20177)}

Immunohistochemistry - ZNF207 Rabbit pAb (A20177)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using ZNF207 Rabbit pAb (A20177) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A20177? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)