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ZNF20 Rabbit pAb (A7346)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF20 Rabbit pAb (A7346)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF20 using ZNF20 Rabbit pAb (A7346) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of paraffin-embedded Human brain cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameZNF20 Rabbit pAb
Catalog No.A7346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ZNF20 (NP_066966.2).
SequenceMMFQDSVAFEDVAVSFTQEEWALLDPSQKNLYRDVMQETFKNLTSVGKTWKVQNIEDEYKNPRRNLSLMREKLCESKESHHCGESFNQIADDMLNRKTLPGITPCESSVCGEVGTGHSSLNTHIRADTGHKSSEYQEYGENPYRNKECKKAFSYLDSFQSHDKACTKEKPYDGKECTETFISHSCIQRHRVMHSGDGPYK
Gene ID
Swiss Prot
SynonymsKOX13; ZNF20
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T transfected with ZNF20
Cellular locationNucleus

Documents

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ABclonal:Western blot - ZNF20 Rabbit pAb (A7346)}

Western blot - ZNF20 Rabbit pAb (A7346)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF20 using ZNF20 Rabbit pAb (A7346) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)}

Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of paraffin-embedded Human brain cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)}

Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of paraffin-embedded Human kidney cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)