제품 > 항체 > 다중클론항체(pAb)

ZNF683 Rabbit pAb (A26154)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ZNF683 Rabbit pAb (A26154)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ZNF683 using ZNF683 Rabbit pAb (A26154) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 10s.

Overview

Product nameZNF683 Rabbit pAb
Catalog No.A26154
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in T cell receptor signaling pathway; cellular response to ionomycin; and regulation of gene expression. Predicted to be located in nucleus.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 420-524 of human ZNF683 (NP_001294854.1).
SequenceHSGARPFQCSVCRSRFTQHIHLKLHHRLHAPQPCGLVHTQLPLASLACLAQWHQGALDLMAVASEKHMGYDIDEVKVSSTSQGKARAVSLSSAGTPLVMGQDQNN
Gene ID
Swiss Prot
SynonymsHobit; ZNF683
Calculated MW57kDa
Observed MW57kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3
Key applicationWestern blotting    
Positive samples293F cells transfected with ZNF683
Cellular locationNucleus.

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    ABclonal:Western blot - ZNF683 Rabbit pAb (A26154)}

    Western blot - ZNF683 Rabbit pAb (A26154)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ZNF683 using ZNF683 Rabbit pAb (A26154) at 1:1000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 20 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020)
    .Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)