제품 > 항체 > 다중클론항체(pAb)

ZmSNF2 Rabbit pAb (A23098)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Maize

ABclonal:Western blot - ZmSNF2 Rabbit pAb (A23098)

Western blot analysis of lysates from wild type (WT) and Maize transfected with ZmSNF2 using ZmSNF2 Rabbit pAb (A23098) at 1:900 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameZmSNF2 Rabbit pAb
Catalog No.A23098
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
These proteins are broadly defined as transcriptional regulators that either act by interacting with transcription factors or as chromatin modifiers restricting or releasing DNA accessibility. Thus, GrassCoRegDB includes proteins with functions such as covalent histone modification (acetylation, methylation, phosphorylation, ubiquitination and sumoylation), as members of the ATP dependent chromatin remodeling complexes, and as histone chaperones, among others. The specific role of many of these proteins in transcription is still unknown. In some cases the community has already set-up some rules for systematic naming of those proteins, in these a protein name is reported in Grassius. For proteins lacking a systematic name, loci identifiers or common names are used instead. As identification of proteins with roles in transcriptional regulation is a growing research field, GrassCoRegDB will be frequently updated to keep pace with the literature. For this set of proteins the same curation efforts as for other Grassius collections are applied and links to other Grassius resources such as the TFome collection are available.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 901-1089 of maize ZmSNF2(XP_020405944.1).
SequenceFSQFLEHIHVIEQQLTIAGIIYSGMYSPMPLASKVGLSESEKRKFWEDLDGMVRAVPTNEKLSVGDLNGHVGSTNAGYELAHGGFGTHRDKQAKIARMKWWKLKGETFEVFRERVFVEGIWFEEDANMWVKMATCIRKVVSKVFGVTKGSSGESKDTWWWTEDVQKAIKEKKKCYRTLFHDNSTVNIER
Gene ID
Swiss Prot
SynonymsGRMZM2G167668; GRMZM2G379804; ZmSNF2
Calculated MW155kDa
Observed MW120kDa
ReactivityMaize
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMaize cells transfected with ZmSNF2
Cellular location

Documents

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Lot number

    ABclonal:Western blot - ZmSNF2 Rabbit pAb (A23098)}

    Western blot - ZmSNF2 Rabbit pAb (A23098)

    Western blot analysis of lysates from wild type (WT) and Maize transfected with ZmSNF2 using ZmSNF2 Rabbit pAb (A23098) at 1:900 dilution. 
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)