제품 > 항체 > 다중클론항체(pAb)

bZIP46 Rabbit pAb (A20627)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - bZIP46 Rabbit pAb (A20627)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using bZIP46 Rabbit pAb (A20627) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

Overview

Product namebZIP46 Rabbit pAb
Catalog No.A20627
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transcription factor involved in abscisic acid (ABA) signaling pathway Transcription factor activity is fully activated by ABA.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 100-200 of Oryza sativa bZIP46. (Q69TW5).
SequenceWRDIMGLGGSDDEDPAAAAAAAAPAQRQPTLGEMTLEEFLVRAGVVREDMGQTIVLPPQAQALFPGSNVVAPAMQLANGMLPGVVGVAPGAAAAMTVAAPA
Gene ID
Swiss Prot
SynonymsP0701E03.2; bZIP46
Calculated MW35kDa
Observed MW50kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesleaves
Cellular locationnucleus
    ABclonal:Western blot - bZIP46 Rabbit pAb (A20627)}

    Western blot - bZIP46 Rabbit pAb (A20627)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using bZIP46 Rabbit pAb (A20627) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 120s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)