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eIF4A Rabbit pAb (A20251)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - eIF4A Rabbit pAb (A20251)

Western blot analysis of various lysates using eIF4A Rabbit pAb (A20251) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)

Immunofluorescence analysis of C6 cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)

Immunofluorescence analysis of NIH-3T3 cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)

Immunofluorescence analysis of U-2 OS cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameeIF4A Rabbit pAb
Catalog No.A20251
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF4A (NP_001407.1).
SequenceMSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKA
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HeLa, HepG2, MCF7, C2C12, Mouse brain, Rat brain
Cellular locationcytoplasm, cytosol, eukaryotic translation initiation factor 4F complex, extracellular exosome.

Documents

Certificate of Compliance

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    ABclonal:Western blot - eIF4A Rabbit pAb (A20251)}

    Western blot - eIF4A Rabbit pAb (A20251)

    Western blot analysis of various lysates using eIF4A Rabbit pAb (A20251) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)}

    Immunofluorescence - eIF4A Rabbit pAb (A20251)

    Immunofluorescence analysis of C6 cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)}

    Immunofluorescence - eIF4A Rabbit pAb (A20251)

    Immunofluorescence analysis of NIH-3T3 cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - eIF4A Rabbit pAb (A20251)}

    Immunofluorescence - eIF4A Rabbit pAb (A20251)

    Immunofluorescence analysis of U-2 OS cells using eIF4A Rabbit pAb (A20251) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)