제품 > 항체 > 단일클론항체(mAb)

p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Western blot analysis of lysates from PC-12 cells using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Western blot analysis of lysates from Mouse brain using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Human colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Overview

Product namep75NTR/NGFR Rabbit PolymAb®
Catalog No.A26533PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human p75NTR/NGFR (NP_150086.2).
SequenceKLHSDSGISVDSQSLHDQQPHTQTASGQALKGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDA
Gene ID
Swiss Prot
SynonymsCD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Calculated MW45kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:10000
  • IHC-P 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, PC-12
Cellular locationMembrane, Single-pass type I membrane protein, Cell surface, cell-cell junction, cytosol, dendritic spine, endosome, extracellular region, growth cone, membrane, neuromuscular junction, nucleoplasm, perikaryon, plasma membrane, postsynaptic density, presynapse.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Western blot analysis of lysates from PC-12 cells using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Western blot - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Western blot analysis of lysates from Mouse brain using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at 1:5000 dilution incubated overnight at 4℃.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Human breast tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Human colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)}

    Immunohistochemistry - p75NTR/NGFR Rabbit PolymAb® (A26533PM)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using p75NTR/NGFR Rabbit PolymAb® (A26533PM) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)