제품 > 항체 > 다중클론항체(pAb)

pAbPC1 Rabbit pAb (A14872)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - pAbPC1 Rabbit pAb (A14872)

Western blot analysis of various lysates, using pAbPC1 Rabbit pAb (A14872) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Rat testis using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of MCF7 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of NIH/3T3 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of PC-12 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product namepAbPC1 Rabbit pAb
Catalog No.A14872
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a poly(A) binding protein. The protein shuttles between the nucleus and cytoplasm and binds to the 3' poly(A) tail of eukaryotic messenger RNAs via RNA-recognition motifs. The binding of this protein to poly(A) promotes ribosome recruitment and translation initiation; it is also required for poly(A) shortening which is the first step in mRNA decay. The gene is part of a small gene family including three protein-coding genes and several pseudogenes.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 350-450 of human PABPC1 (NP_002559.2).
SequenceVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNM
Gene ID
Swiss Prot
SynonymsPAB1; PABP; PABP1; PABPC2; PABPL1; pAbPC1
Calculated MW71kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, PC-12
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Homo sapiens)

IF(Homo sapiens)

IF(Homo sapiens)

IP(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - pAbPC1 Rabbit pAb (A14872)}

Western blot - pAbPC1 Rabbit pAb (A14872)

Western blot analysis of various lysates, using pAbPC1 Rabbit pAb (A14872) at 1:900 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)}

Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Rat testis using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)}

Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)}

Immunohistochemistry - pAbPC1 Rabbit pAb (A14872)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)}

Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of MCF7 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)}

Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of NIH/3T3 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - pAbPC1 Rabbit pAb (A14872)}

Immunofluorescence - pAbPC1 Rabbit pAb (A14872)

Immunofluorescence analysis of PC-12 cells using pAbPC1 Rabbit pAb (A14872) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A14872? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)