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ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)

Flow cytometry:1X10^6 K562 cells(negative control, left) and U937 cells(right) were surface-stained with ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb(A23698, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)

Flow cytometry:1X10^6 U937 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb(A23698, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb
Catalog No.A23698
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60966-ABf488
This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-434 of human CD170/Siglec-5 (NP_003821.1).
SequenceEKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGT
Gene ID
Swiss Prot
SynonymsCD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Calculated MW61kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein, Plasma membrane

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)

Flow cytometry:1X10^6 K562 cells(negative control, left) and U937 cells(right) were surface-stained with ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb(A23698, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb (A23698)

Flow cytometry:1X10^6 U937 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD170/Siglec-5 mAb(A23698, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)