제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and U-937 cells (right) were surface-stained with PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 U-937 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119, 5 μl/Test, right).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product name	PE Rabbit anti-Human CD170/Siglec-5 mAb
Catalog No.	A26119
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60966-PE

Background

This gene encodes a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. These cell surface lectins are characterized by structural motifs in the immunoglobulin (Ig)-like domains and sialic acid recognition sites in the first Ig V set domain. The encoded protein is a member of the CD33-related subset of Siglecs and inhibits the activation of several cell types including monocytes, macrophages and neutrophils. Binding of group B Streptococcus (GBS) to the encoded protein plays a role in GBS immune evasion.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 17-434 of human CD170/Siglec-5 (NP_003821.1).
Sequence	EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGT
Gene ID
Swiss Prot
Synonyms	CD170; OBBP2; CD33L2; OB-BP2; SIGLEC-5
Calculated MW	61kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein, Plasma membrane.

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

PE Rabbit anti-Human CD170/Siglec-5 mAb images

Flow CytoMetry - PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119)

Flow cytometry: 1X10^6 U-937 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Human CD170/Siglec-5 mAb (A26119)

* For research use only. Not for therapeutic or diagnostic purposes.