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ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 MOLT-4 cells (Low Expression, left) and A-431 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 A431 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 MOLT-4 cells(Low Expression) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 Human PBMC were intracellularly-stained with ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 Human PBMC cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human CD68 mAb
Catalog No.A22514
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56633-ABf488
This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-319 of human CD68 (NP_001242.2).
SequenceNDCPHKKSATLLPSFTVTPTVTESTGTTSHRTTKSHKTTTHRTTTTGTTSHGPTTATHNPTTTSHGNVTVHPTSNSTATSQGPSTATHSPATTSHGNATVHPTSNSTATSPGFTSSAHPEPPPPSPSPSPTSKETIGDYTWTNGSQPCVHLQAQIQIRVMYTTQGGGEAWGISVLNPNKTKVQGSCEGAHPHLLLSFPYGHLSFGFMQDLQQKVVYLSYMAVEYNVSFPHAAQWTFSAQNASLRDLQAPLGQSFSCSNSSIILSPAVHLDLLSLRLQAAQLPHTGVFGQSFSCPSDRS
Gene ID
Swiss Prot
SynonymsGP110; LAMP4; SCARD1
Calculated MW37kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein, Endosome membrane, Lysosome membrane

Documents

Certificate of Compliance

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Lot number

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 MOLT-4 cells (Low Expression, left) and A-431 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 A431 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 MOLT-4 cells(Low Expression) were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 Human PBMC were intracellularly-stained with ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line).Non-fluorescently stained Human PBMC was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human CD68 mAb (A22514)

Flow cytometry:1X10^6 Human PBMC cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human CD68 mAb(A22514, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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