제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human Calreticulin mAb (A27387)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 knockout (KO) HeLa cells (negative control, left) and HeLa cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Human Calreticulin mAb (A27387, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HeLa cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human Calreticulin mAb (A27387, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human Calreticulin mAb
Catalog No.	A27387
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC71596-ABflo488

Background

Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 335-417 of human Calreticulin (NP_004334.1).
Sequence	DEAYAEEFGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDKDEDEEDEEDKEEDEEEDVPGQAKDEL
Gene ID
Swiss Prot
Synonyms	RO; CRT; SSA; cC1qR; HEL-S-99n
Calculated MW	48kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location	Cell surface, Cytoplasm, Endoplasmic reticulum lumen, Sarcoplasmic reticulum lumen, Secreted, cytosol, extracellular matrix, extracellular space, cell surface, cytolytic granule, cytoplasm, endoplasmic reticulum, endoplasmic reticulum lumen, endoplasmic reticulum quality control compartment, external side of plasma membrane, extracellular exosome, extracellular region, focal adhesion, Golgi apparatus, nuclear envelope, nucleus, perinuclear region of cytoplasm, smooth endoplasmic reticulum.