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ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)

Flow cytometry:1X10^6 Daudi cells (negative control, Left) and MCF7 cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb(A24357, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgM isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)

Flow cytometry:1X10^6 MCF7 cells were surface-stained with ABflo® 488 Rabbit IgM isotype control (5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb(A24357, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb
Catalog No.A24357
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgM
CloneNo.ARC55156-IgM-01-ABf488
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-643 of human ErbB3/HER3 (NP_001973.2).
SequenceSEVGNSQAVCPGTLNGLSVTGDAENQYQTLYKLYERCEVVMGNLEIVLTGHNADLSFLQWIREVTGYVLVAMNEFSTLPLPNLRVVRGTQVYDGKFAIFVMLNYNTNSSHALRQLRLTQLTEILSGGVYIEKNDKLCHMDTIDWRDIVRDRDAEIVVKDNGRSCPPCHEVCKGRCWGPGSEDCQTLTKTICAPQCNGHCFGPNPNQCCHDECAGGCSGPQDTDCFACRHFNDSGACVPRCPQPLVYNKLTFQLEPNPHTKYQYGGVCVASCPHNFVVDQTSCVRACPPDKMEVDKNGLKMCEPCGGLCPKACEGTGSGSRFQTVDSSNIDGFVNCTKILGNLDFLITGLNGDPWHKIPALDPEKLNVFRTVREITGYLNIQSWPPHMHNFSVFSNLTTIGGRSLYNRGFSLLIMKNLNVTSLGFRSLKEISAGRIYISANRQLCYHHSLNWTKVLRGPTEERLDIKHNRPRRDCVAEGKVCDPLCSSGGCWGPGPGQCLSCRNYSRGGVCVTHCNFLNGEPREFAHEAECFSCHPECQPMEGTATCNGSGSDTCAQCAHFRDGPHCVSSCPHGVLGAKGPIYKYPDVQNECRPCHENCTQGCKGPELQDCLGQTLVLIGKTHLT
Gene ID
Swiss Prot
SynonymsERBB3; ErbB-3; HER3; LCCS2; MDA-BF-1; c-erbB-3; c-erbB3; erbB3-S; p180-ErbB3; p45-sErbB3; p85-sErbB3; erb-b2 receptor tyrosine kinase 3
Calculated MW20kDa/36kDa/77kDa/141kDa/148kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein

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ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)

Flow cytometry:1X10^6 Daudi cells (negative control, Left) and MCF7 cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb(A24357, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgM isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)}

Flow CytoMetry - ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb (A24357)

Flow cytometry:1X10^6 MCF7 cells were surface-stained with ABflo® 488 Rabbit IgM isotype control (5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ErbB3/HER3 mAb(A24357, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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