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Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052)

Western blot analysis of extracts of 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

Overview

Product namePhospho-ErbB3/HER3-Y1222 Rabbit mAb
Catalog No.AP1052
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1599
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
ImmunogenA synthetic phosphorylated peptide around Y1222 of human ERBB3 (P21860).
SequenceLGYEY
Gene ID
Swiss Prot
SynonymsHER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
Calculated MW148kDa
Observed MW185kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein

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ABclonal:Western blot - Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052)}

Western blot - Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052)

Western blot analysis of extracts of 293T cells, using Phospho-ErbB3/HER3-Y1222 Rabbit mAb (AP1052) at 1:1000 dilution.293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)