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[KO Validated] ErbB3/HER3 Rabbit pAb (A0950)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)

Western blot analysis of various lysates using [KO Validated] ErbB3/HER3 Rabbit pAb (A0950) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)

Western blot analysis of lysates from wild type (WT) and ErbB3/HER3 knockout (KO) 293T cells, using [KO Validated] ErbB3/HER3 Rabbit pAb (A0950) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name[KO Validated] ErbB3/HER3 Rabbit pAb
Catalog No.A0950
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1275-1342 of human ErbB3/HER3 (NP_001973.2).
SequenceDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT
Gene ID
Swiss Prot
SynonymsHER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3; R3
Calculated MW148kDa
Observed MW185kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, MCF7, A-431, Mouse liver
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)}

Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)

Western blot analysis of various lysates using [KO Validated] ErbB3/HER3 Rabbit pAb (A0950) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)}

Western blot - [KO Validated] ErbB3/HER3 Rabbit pAb (A0950)

Western blot analysis of lysates from wild type (WT) and ErbB3/HER3 knockout (KO) 293T cells, using [KO Validated] ErbB3/HER3 Rabbit pAb (A0950) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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