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ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)

Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)

Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794, 5 μl/Test, right).

Overview

Product nameABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb
Catalog No.A26794
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC69845-ABflo488
Predicted to enable integrin binding activity. Acts upstream of or within phagocytosis. Located in plasma membrane. Is expressed in brain. Orthologous to human ICAM5 (intercellular adhesion molecule 5).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-227 of mouse CD50/ICAM-3 (NP_032345.2).
SequenceEPFWADLQPRVALVERGGSLWLNCSTNCPRPERGGLETSLRRNGTQRGLRWLARQLVDIREPETQPVCFFRCARRTLQARGLIRTFQRPDRVELVPLPSWQPVGENFTLSCRVPGAGPRASLTLTLLRGGQELIRRSFVGEPPRARGAMLTARVLARREDHRVNFSCLAELDLRPHGLGLFANSSAPRQLRTFAMP
Gene ID
Swiss Prot
SynonymsTLN; CD50; Tlcn; Icam3; ICAM-3; ICAM-5
Calculated MW97kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein.

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    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)

    Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794)

    Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD50/ICAM-3 mAb (A26794, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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