제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse Ki67 mAb (A26239)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 C57BL/6 mouse splenocytes (Low Expression, left) and RAW 264.7 cells (right) were intracellularly-stained with ABflo® 488 Rabbit anti-Mouse Ki67 mAb (A26239, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 RAW 264.7 cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse Ki67 mAb (A26239, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse Ki67 mAb
Catalog No.	A26239
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC69121-ABflo488

Background

Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucl

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1816-2163 of mouse Ki67 (NP_001074586.2).
Sequence	DETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP
Gene ID
Swiss Prot
Synonyms	Ki67; Ki-67; D630048A14Rik
Calculated MW	351kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location	Nucleus, Nucleus, nucleolus.