Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat
Western blot analysis of lysates from RAW 264.7 cells using Ki67 Rabbit PolymAb® (A26419PM) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using Ki67 Rabbit PolymAb® (A26419PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Ki67 Rabbit PolymAb® (A26419PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Ki67 Rabbit PolymAb® (A26419PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Ki67 Rabbit PolymAb® (A26419PM) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Rat spleen tissue using Ki67 Rabbit PolymAb® (A26419PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse spleen tissue using Ki67 Rabbit PolymAb® (A26419PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of RAW 264.7 cells using Ki67 Rabbit PolymAb® (A26419PM, dilution 1:800) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of NIH/3T3 cells using Ki67 Rabbit PolymAb® (A26419PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
| Product name | Ki67 Rabbit PolymAb® |
|---|---|
| Catalog No. | A26419PM |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1816-2163 of mouse Ki67 (NP_001074586.2). |
|---|---|
| Sequence | DETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Ki67; Ki-67; D630048A14Rik |
| Calculated MW | 351kDa |
| Observed MW | 359kDa以下 |
| Reactivity | Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | RAW 264.7 |
| Cellular location | Nucleus, Nucleus, nucleolus. |
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