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Mki67 Rabbit pAb (A25399)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse, Rat

ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Mki67 Rabbit pAb (A25399)

Immunofluorescence analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - Mki67 Rabbit pAb (A25399)

Immunofluorescence analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameMki67 Rabbit pAb
Catalog No.A25399
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein C-terminus binding activity. Involved in regulation of chromatin organization. Acts upstream of or within cholangiocyte proliferation; hepatocyte proliferation; and meiotic cell cycle. Located in chromosome; cytoplasm; and nucleolus. Is expressed in several structures, including alimentary system; brain; genitourinary system; sensory organ; and skin. Human ortholog(s) of this gene implicated in Crohn's disease; breast cancer; human immunodeficiency virus infectious disease; and pancreatic cancer. Orthologous to human MKI67 (marker of proliferation Ki-67).
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 1816-2163 of mouse Mki67 (NP_001074586.2).
SequenceDETTKIALQSPQPGHIINPASMKRQSNMSLRKDMREFSILEKQTQSRGRDAGTPAPMQEENGTTAIMETPKQKLDFIGNSTGHKRRPRTPKNRAQPLEDLDGFQELFQTPAGASDPVSVEESAKISLASSQAEPVRTPASTKRRSKTGLSKVDVRQEPSTLGKRMKSLGRAPGTPAPVQEENDSTAFMETPKQKLDFTGNSSGHKRRPQTPKIRAQPLEDLDGFQELFQTPAGANDSVTVEESVKMSLESSQAEPVKTPASTKRLSKTGLSKVDVREDPSILEKKTKSPGTPAPVQEENDCTAFMETPKQKLDFTGNSSGHKRRPRTPKIRAQPLEDLDGFQELFQTP
Gene ID
Swiss Prot
SynonymsKi67; Ki-67; D630048A14Rik
Calculated MW350kDa
Observed MWRefer to figures
ReactivityMouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationNucleus
Customer validation

IHC(Mus musculus, Rattus norvegicus)

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)}

    Immunohistochemistry - Mki67 Rabbit pAb (A25399)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)}

    Immunohistochemistry - Mki67 Rabbit pAb (A25399)

    Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)}

    Immunohistochemistry - Mki67 Rabbit pAb (A25399)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)}

    Immunohistochemistry - Mki67 Rabbit pAb (A25399)

    Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - Mki67 Rabbit pAb (A25399)}

    Immunohistochemistry - Mki67 Rabbit pAb (A25399)

    Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - Mki67 Rabbit pAb (A25399)}

    Immunofluorescence - Mki67 Rabbit pAb (A25399)

    Immunofluorescence analysis of paraffin-embedded Mouse spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
    ABclonal:Immunofluorescence - Mki67 Rabbit pAb (A25399)}

    Immunofluorescence - Mki67 Rabbit pAb (A25399)

    Immunofluorescence analysis of paraffin-embedded Rat spleen tissue using Mouse Mki67 Rabbit pAb (A25399) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    항체 (3)

    Secondary Antibodies (26)