제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human CD271/NGFR mAb (A23119)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 K-562 cells (negative control, Left) and SK-N-MC cells (Right) were surface-stained with ABflo® 594 Rabbit anti-Human NGFR/CD271 mAb(A23119, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 SK-N-MC cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Human NGFR/CD271 mAb(A23119, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human CD271/NGFR mAb
Catalog No.	A23119
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57800-ABf594

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 29-250 of human CD271/NGFR (NP_002498.1).
Sequence	KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN
Gene ID
Swiss Prot
Synonyms	CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Calculated MW	35kDa/45kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

ABflo® 594 Rabbit anti-Human CD271/NGFR mAb images

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD271/NGFR mAb (A23119)

Flow cytometry:1X10^6 SK-N-MC cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Human NGFR/CD271 mAb(A23119, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.