제품 > 항체 > 다중클론항체(pAb)

CD271 Rabbit pAb (A25235)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Immunohistochemistry analysis of CD271 in paraffin-embedded human tonsil tissue using CD271 Rabbit pAb (A25235) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

Overview

Product name	CD271 Rabbit pAb
Catalog No.	A25235
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Immunogen information

Immunogen	Recombinant Protein corresponding to a sequence within amino acids 330-427 of human CD271 (NP_002498.1).
Sequence	KGDGGLYSSLPPAKREEVEKLLNGSAGDTWRHLAGELGYQPEHIDSFTHEACPVRALLASWATQDSATLDALLAALRRIQRADLVESLCSESTATSPV
Gene ID
Swiss Prot
Synonyms	CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR; p75NTR/NGFR
Calculated MW	45kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Immunohistochemistry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein