제품 > 항체 > 단일클론항체(mAb)

ABflo® 700 Rabbit anti-Human CD271/NGFR mAb (A26369)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and SK-N-MC cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human NGFR/CD271 mAb (A26369, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 SK-N-MC cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human NGFR/CD271 mAb (A26369, 5 μl/Test, right).

Overview

Product name	ABflo® 700 Rabbit anti-Human CD271/NGFR mAb
Catalog No.	A26369
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57800-ABflo700

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 29-250 of human CD271/NGFR (NP_002498.1).
Sequence	KEACPTGLYTHSGECCKACNLGEGVAQPCGANQTVCEPCLDSVTFSDVVSATEPCKPCTECVGLQSMSAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLVFSCQDKQNTVCEECPDGTYSDEANHVDPCLPCTVCEDTERQLRECTRWADAECEEIPGRWITRSTPPEGSDSTAPSTQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDN
Gene ID
Swiss Prot
Synonyms	CD271; p75NTR; TNFRSF16; p75(NTR); Gp80-LNGFR
Calculated MW	35kDa/45kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.