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ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)

Flow cytometry:1X10^6 HepG2 cells (Low Expression, Left) and MOLT-4 cells(Right) were surface-stained with ABflo® 594 Rabbit anti-Human CD47 mAb(A24302, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD47 mAb(A24302, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human CD47 mAb
Catalog No.A24302
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61212-ABflo594
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 19-139 of human CD47 (NP_001768.1).
SequenceQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP
Gene ID
Swiss Prot
SynonymsCD47; IAP; MER6; OA3; CD47 molecule
Calculated MW31kDa/33kDa/35kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Multi-pass membrane protein

Documents

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)

Flow cytometry:1X10^6 HepG2 cells (Low Expression, Left) and MOLT-4 cells(Right) were surface-stained with ABflo® 594 Rabbit anti-Human CD47 mAb(A24302, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD47 mAb (A24302)

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD47 mAb(A24302, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (5)

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