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ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of Hep G2 cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of Jurkat cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of MOLT-4 cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Flow cytometry: 1X10^6 U266 cells (negative control, left) and Jurkat cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Flow cytometry: 1X10^6 Jurkat cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human CD99 mAb
Catalog No.A25833
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67259-ABflo594
The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 24-123 of human CD99 (NP_002405.1).
SequenceGGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADA
Gene ID
Swiss Prot
SynonymsMIC2; HBA71; MIC2X; MIC2Y; MSK5X
Calculated MW19kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationcytoplasm, focal adhesion, plasma membrane, Membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)}

Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of Hep G2 cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)}

Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of Jurkat cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)}

Immunofluorescence - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Confocal imaging of MOLT-4 cells using ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, dilution 1:25) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Flow cytometry: 1X10^6 U266 cells (negative control, left) and Jurkat cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD99 mAb (A25833)

Flow cytometry: 1X10^6 Jurkat cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD99 mAb (A25833, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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