제품 > 항체 > 단일클론항체(mAb)

FITC Rabbit anti-Human CD99 mAb (A26936)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 U266 cells (negative control, left) and Human PBMC (right) were surface-stained with FITC Rabbit anti-Human CD99 mAb (A26936, 5 μl/Test, orange line) or FITC Rabbit IgG isotype control (A25616, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with FITC Rabbit IgG isotype control (A25616, 5 μl/Test, left) or FITC Rabbit anti-Human CD99 mAb (A26936, 5 μl/Test, right).

Overview

Product name	FITC Rabbit anti-Human CD99 mAb
Catalog No.	A26936
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67259-FITC

Background

The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 24-123 of human CD99 (NP_002405.1).
Sequence	GGFDLSDALPDNENKKPTAIPKKPSAGDDFDLGDAVVDGENDDPRPPNPPKPMPNPNPNHPSSSGSFSDADLADGVSGGEGKGGSDGGGSHRKEGEEADA
Gene ID
Swiss Prot
Synonyms	MIC2; HBA71; MIC2X; MIC2Y; MSK5X
Calculated MW	19kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	cytoplasm, focal adhesion, plasma membrane, Membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

FITC Rabbit anti-Human CD99 mAb images

Flow CytoMetry - FITC Rabbit anti-Human CD99 mAb (A26936)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with FITC Rabbit IgG isotype control (A25616, 5 μl/Test, left) or FITC Rabbit anti-Human CD99 mAb (A26936, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.