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ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)

Flow cytometry: 1X10^6 Reh cells (Low Expression, left) and Caco-2 (right) cells were intracellularly-stained with ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb(A24717, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)

Flow cytometry: 1X10^6 Caco-2 cells were intracellularly-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb
Catalog No.A24717
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63830-ABflo594
The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human Megalin/LRP2 (NP_004516.2).
SequenceFHYRRTGSLLPALPKLPSLSSLVKPSENGNGVTFRSGADLNMDIGVSGFGPETAIDRSMAMSEDFVMEMGKQPIIFENPMYSARDSAVKVVQPIQVTVSENVDNKNYGSPINPSEIVPETNPTSPAADGTQVTKWNLFKRKSKQTTNFENPIYAQMENEQKESVAATPPPSPSLPAKPKPPSRRDPTPTYSATEDTFKDTANLVKEDSEV
Gene ID
Swiss Prot
SynonymsLRP2; DBS; GP330; LDL receptor related protein 2
Calculated MW521kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC (intra) 5μl per 10^6 cells in 100 μl volume.
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular locationMembrane, Single-pass type I membrane protein, coated pit

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)

Flow cytometry: 1X10^6 Reh cells (Low Expression, left) and Caco-2 (right) cells were intracellularly-stained with ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb(A24717, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717)

Flow cytometry: 1X10^6 Caco-2 cells were intracellularly-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human Megalin/LRP2 mAb (A24717, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)