제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Confocal imaging of paraffin-embedded Mouse kidney tissue using ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Confocal imaging of paraffin-embedded Rat kidney tissue using ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716, dilution 1:100). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716)

Flow cytometry: 1X10^6 Reh cells (Low Expression, left) and Caco-2 (right) cells were intracellularly-stained with ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb(A24716, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells was used as blank control (red line).

Flow cytometry: 1X10^6 Caco-2 cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb (A24716, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human Megalin/LRP2 mAb
Catalog No.	A24716
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63830-ABflo647

Background

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human Megalin/LRP2 (NP_004516.2).
Sequence	FHYRRTGSLLPALPKLPSLSSLVKPSENGNGVTFRSGADLNMDIGVSGFGPETAIDRSMAMSEDFVMEMGKQPIIFENPMYSARDSAVKVVQPIQVTVSENVDNKNYGSPINPSEIVPETNPTSPAADGTQVTKWNLFKRKSKQTTNFENPIYAQMENEQKESVAATPPPSPSLPAKPKPPSRRDPTPTYSATEDTFKDTANLVKEDSEV
Gene ID
Swiss Prot
Synonyms	LRP2; DBS; GP330; LDL receptor related protein 2
Calculated MW	521kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC (intra) 5μl per 10^6 cells in 100 μl volume.
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein, coated pit.