제품 > 항체 > 단일클론항체(mAb)

Megalin/LRP2 Rabbit mAb (A24690)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse kidney using Megalin/LRP2 Rabbit mAb(A24690) at 1:1000 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:20s.

ABclonal:Immunohistochemistry - Megalin/LRP2 Rabbit mAb (A24690)

Immunohistochemistry analysis of Megalin/LRP2 in paraffin-embedded human kidney using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Megalin/LRP2 in paraffin-embedded human liver using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Megalin/LRP2 in paraffin-embedded mouse kidney using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of Megalin/LRP2 in paraffin-embedded rat liver using Megalin/LRP2 Rabbit mAb (A24690) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Megalin/LRP2 Rabbit mAb (A24690)

Confocal imaging of mouse kidney using Megalin/LRP2 Rabbit mAb (A24690, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Confocal imaging of rat kidney using Megalin/LRP2 Rabbit mAb (A24690, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

ABclonal:Flow CytoMetry - Megalin/LRP2 Rabbit mAb (A24690)

Flow cytometry: 1X10^6 Reh cells (Low Expression, left) and Caco-2 (right) cells were intracellularly-stained with Megalin/LRP2 Rabbit mAb (A24690, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Caco-2 cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or Megalin/LRP2 Rabbit mAb (A24690, 2 μg/mL, right).

Overview

Product name	Megalin/LRP2 Rabbit mAb
Catalog No.	A24690
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63830

Background

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 4446-4655 of human Megalin/LRP2（NP_004516.2）.
Sequence	FHYRRTGSLLPALPKLPSLSSLVKPSENGNGVTFRSGADLNMDIGVSGFGPETAIDRSMAMSEDFVMEMGKQPIIFENPMYSARDSAVKVVQPIQVTVSENVDNKNYGSPINPSEIVPETNPTSPAADGTQVTKWNLFKRKSKQTTNFENPIYAQMENEQKESVAATPPPSPSLPAKPKPPSRRDPTPTYSATEDTFKDTANLVKEDSEV
Gene ID
Swiss Prot
Synonyms	DBS; GP330; LRP-2; Megalin/LRP2
Calculated MW	522kDa
Observed MW	250kDa+

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 FC (intra) 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse kidney
Cellular location	Membrane, Single-pass type I membrane protein, coated pit

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Megalin/LRP2 Rabbit mAb images

Western blot - Megalin/LRP2 Rabbit mAb (A24690)

Immunohistochemistry - Megalin/LRP2 Rabbit mAb (A24690)

Immunofluorescence - Megalin/LRP2 Rabbit mAb (A24690)

Confocal imaging of mouse kidney using Megalin/LRP2 Rabbit mAb (A24690, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Immunofluorescence - Megalin/LRP2 Rabbit mAb (A24690)

Confocal imaging of rat kidney using Megalin/LRP2 Rabbit mAb (A24690, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 40x.

Flow CytoMetry - Megalin/LRP2 Rabbit mAb (A24690)

Flow cytometry: 1X10^6 Caco-2 cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or Megalin/LRP2 Rabbit mAb (A24690, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.