제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD24 mAb (A25381)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Confocal imaging of MCF7 cells using ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD24 mAb (A25381)

Flow cytometry: 1X10^6 293F cells (Low Expression, left) and MCF7 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 MCF7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human CD24 mAb
Catalog No.	A25381
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66763-ABflo647

Background

This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-80 of human CD24 (NP_037362.1).
Sequence	MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS
Gene ID
Swiss Prot
Synonyms	CD24A; CD24
Calculated MW	8kDa; 13kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Cell membrane, GPI-anchor, Lipid-anchor

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

ABflo® 647 Rabbit anti-Human CD24 mAb images

Immunofluorescence - ABflo® 647 Rabbit anti-Human CD24 mAb (A25381)

Confocal imaging of MCF7 cells using ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, dilution 1:100). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD24 mAb (A25381)

Flow cytometry: 1X10^6 MCF7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD24 mAb (A25381, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.