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ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb
Catalog No.A26591
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC69331-ABflo647
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-615 of human Desmoglein 3/PVA (NP_001935.2).
SequenceEWVKFAKPCREGEDNSKRNPIAKITSDYQATQKITYRISGVGIDQPPFGIFVVDKNTGDINITAIVDREETPSFLITCRALNAQGLDVEKPLILTVKILDINDNPPVFSQQIFMGEIEENSASNSLVMILNATDADEPNHLNSKIAFKIVSQEPAGTPMFLLSRNTGEVRTLTNSLDREQASSYRLVVSGADKDGEGLSTQCECNIKVKDVNDNFPMFRDSQYSARIEENILSSELLRFQVTDLDEEYTDNWLAVYFFTSGNEGNWFEIQTDPRTNEGILKVVKALDYEQLQSVKLSIAVKNKAEFHQSVISRYRVQSTPVTIQVINVREGIAFRPASKTFTVQKGISSKKLVDYILGTYQAIDEDTNKAASNVKYVMGRNDGGYLMIDSKTAEIKFVKNMNRDSTFIVNKTITAEVLAIDEYTGKTSTGTVYVRVPDFNDNCPTAVLEKDAVCSSSPSVVVSARTLNNRYTGPYTFALEDQPVKLPAVWSITTLNATSALLRAQEQIPPGVYHISLVLTDSQNNRCEMPRSLTLEVCQCDNRGICGTSYPTTSPGTRYGRPHSGR
Gene ID
Swiss Prot
SynonymsPVA; ABOLM; CDHF6
Calculated MW108kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell junction, Cell membrane, Single-pass type I membrane protein, desmosome.

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ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591)

Flow cytometry: 1X10^6 A-431 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human Desmoglein 3/PVA mAb (A26591, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (5)

Secondary Antibodies (26)