제품 > 항체 > 단일클론항체(mAb)

Desmoglein 3 Rabbit mAb (A5227)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of extracts of various cell lines, using Desmoglein 3 Rabbit mAb (A5227) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.

ABclonal:Immunofluorescence - Desmoglein 3 Rabbit mAb (A5227)

Immunofluorescence analysis of NIH-3T3 cells using Desmoglein 3 Rabbit mAb (A5227) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	Desmoglein 3 Rabbit mAb
Catalog No.	A5227
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1190

Background

This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. Desmogleins are calcium-binding transmembrane glycoprotein components of desmosomes, cell-cell junctions between epithelial, myocardial, and other cell types. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This gene is present in a gene cluster with other desmoglein gene family members on chromosome 18. The encoded protein has been identified as the autoantigen of the autoimmune blistering disease pemphigus vulgaris.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 900-999 of human Desmoglein 3 (P32926).
Sequence	TLSGSQGASALSTSGSVQPAVSIPDPLQHGNYLVTETYSASGSLVQPSTAGFDPLLTQNVIVTERVICPISSVPGNLAGPTQLRGSHTMLCTEDPCSRLI
Gene ID
Swiss Prot
Synonyms	PVA; ABOLM; CDHF6
Calculated MW	108kDa
Observed MW	145kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	A-431, Cal-27
Cellular location	Cell junction, Cell membrane, Single-pass type I membrane protein, desmosome
Customer validation	IF(Ovis aries)