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ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)

Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and RAW 264.7 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)

Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Mouse CD14 mAb
Catalog No.A25191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66543-ABflo647
This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-345 of mouse CD14 (NP_033971.1).
SequenceAPPEPCELDEESCSCNFSDPKPDWSSAFNCLGAADVELYGGGRSLEYLLKRVDTEADLGQFTDIIKSLSLKRLTVRAARIPSRILFGALRVLGISGLQELTLENLEVTGTAPPPLLEATGPDLNILNLRNVSWATRDAWLAELQQWLKPGLKVLSIAQAHSLNFSCEQVRVFPALSTLDLSDNPELGERGLISALCPLKFPTLQVLALRNAGMETPSGVCSALAAARVQLQGLDLSHNSLRDAAGAPSCDWPSQLNSLNLSFTGLKQVPKGLPAKLSVLDLSYNRLDRNPSPDELPQVGNLSLKGNPFLDSESHSEKFNSGVVTAGAP
Gene ID
Swiss Prot
SynonymsCD14
Calculated MW39kDa
Observed MWRefer to figures
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Golgi apparatus, Secreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)

    Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and RAW 264.7 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191)

    Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse CD14 mAb (A25191, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (4)

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