제품 > 항체 > 단일클론항체(mAb)

APC Rabbit anti-Mouse CD14 mAb (A26055)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Mouse CD14 mAb (A26055, 5 μl/Test, right).

Flow cytometry: 1X10^6 Neuro-2a cells (negative control, left) and RAW 264.7 cells (right) were surface-stained with APC Rabbit anti-Mouse CD14 mAb (A26055, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product name	APC Rabbit anti-Mouse CD14 mAb
Catalog No.	A26055
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66543-APC

Background

This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 18-345 of mouse CD14 (NP_033971.1).
Sequence	APPEPCELDEESCSCNFSDPKPDWSSAFNCLGAADVELYGGGRSLEYLLKRVDTEADLGQFTDIIKSLSLKRLTVRAARIPSRILFGALRVLGISGLQELTLENLEVTGTAPPPLLEATGPDLNILNLRNVSWATRDAWLAELQQWLKPGLKVLSIAQAHSLNFSCEQVRVFPALSTLDLSDNPELGERGLISALCPLKFPTLQVLALRNAGMETPSGVCSALAAARVQLQGLDLSHNSLRDAAGAPSCDWPSQLNSLNLSFTGLKQVPKGLPAKLSVLDLSYNRLDRNPSPDELPQVGNLSLKGNPFLDSESHSEKFNSGVVTAGAP
Gene ID
Swiss Prot
Synonyms	CD14
Calculated MW	39kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Golgi apparatus, Secreted.

Documents

Certificate of Compliance

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Lot number

Research Area

APC Rabbit anti-Mouse CD14 mAb images

Flow CytoMetry - APC Rabbit anti-Mouse CD14 mAb (A26055)

Flow cytometry: 1X10^6 RAW 264.7 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Mouse CD14 mAb (A26055, 5 μl/Test, right).

Flow CytoMetry - APC Rabbit anti-Mouse CD14 mAb (A26055)

* For research use only. Not for therapeutic or diagnostic purposes.