제품 > 항체 > 단일클론항체(mAb)

ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product nameABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb
Catalog No.A26237
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60703-ABflo700
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-458 of human CD85j/ILT2 (NP_006660.4).
SequenceGHLPKPTLWAEPGSVITQGSPVTLRCQGGQETQEYRLYREKKTALWITRIPQELVKKGQFPIPSITWEHAGRYRCYYGSDTAGRSESSDPLELVVTGAYIKPTLSAQPSPVVNSGGNVILQCDSQVAFDGFSLCKEGEDEHPQCLNSQPHARGSSRAIFSVGPVSPSRRWWYRCYAYDSNSPYEWSLPSDLLELLVLGVSKKPSLSVQPGPIVAPEETLTLQCGSDAGYNRFVLYKDGERDFLQLAGAQPQAGLSQANFTLGPVSRSYGGQYRCYGAHNLSSEWSAPSDPLDILIAGQFYDRVSLSVQPGPTVASGENVTLLCQSQGWMQTFLLTKEGAADDPWRLRSTYQSQKYQAEFPMGPVTSAHAGTYRCYGSQSSKPYLLTHPSDPLELVVSGPSGGPSSPTTGPTSTSGPEDQPLTPTGSDPQSGLGRH
Gene ID
Swiss Prot
SynonymsILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B
Calculated MW49kDa/70kDa/71kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCytoplasm, external side of plasma membrane, extracellular region, plasma membrane.

Documents

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ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237)}

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Daudi cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD85j/ILT2 mAb (A26237, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

* For research use only. Not for therapeutic or diagnostic purposes.

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