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LILRB1/CD85j/ILT2 Rabbit mAb (A23528)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using LILRB1/CD85j/ILT2 Rabbit mAb (A23528) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Flow CytoMetry - LILRB1/CD85j/ILT2 Rabbit mAb (A23528)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Daudi cells (right) were surface-stained with LILRB1/CD85j/ILT2 Rabbit mAb (A23528, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Donkey anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Daudi cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or LILRB1/CD85j/ILT2 Rabbit mAb (A23528, 2 μg/mL, right), followed by PE Donkey anti-Rabbit pAb staining.

Overview

Product name	LILRB1/CD85j/ILT2 Rabbit mAb
Catalog No.	A23528
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60700

Background

This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant protein of human LILRB1/CD85j/ILT2.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	ILT2; LIR1; MIR7; PIRB; CD85J; ILT-2; LIR-1; MIR-7; PIR-B; LILRB1/CD85j/ILT2
Calculated MW	49KDa/70KDa/71KDa
Observed MW	110kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 FC 1:500-1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	THP-1
Cellular location	Membrane, Secreted, Single-pass type I membrane protein.