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APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and Daudi (right) cells were surface-stained with APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right).

ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right).

ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 594 Mouse anti-Human CD19 mAb (5 μl/Test) and APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right). Cells in the lymphocyte gate were used for analysis.

Overview

Product nameAPC Rabbit anti-Human CD185/CXCR5 mAb
Catalog No.A26890
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62172-APC
This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-47 of Human CD185/CXCR5 (NP_033981.2).
SequenceMNYPLTLEMDLENLEDLFWELDRLDNYNDTSLVENHLCPATEGPLMA
Gene ID
Swiss Prot
SynonymsBLR1; CD185; MDR15
Calculated MW42kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Multi-pass membrane protein.

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ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)}

Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 HeLa cells (negative control, left) and Daudi (right) cells were surface-stained with APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)}

Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right).
ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)}

Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right).
ABclonal:Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)}

Flow CytoMetry - APC Rabbit anti-Human CD185/CXCR5 mAb (A26890)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 594 Mouse anti-Human CD19 mAb (5 μl/Test) and APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD185/CXCR5 mAb (A26890, 5 μl/Test, right). Cells in the lymphocyte gate were used for analysis.

* For research use only. Not for therapeutic or diagnostic purposes.

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