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CXCR5 Rabbit pAb (A5268)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CXCR5 Rabbit pAb (A5268)

Western blot analysis of extracts of various cell lines, using CXCR5 antibody (A5268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product nameCXCR5 Rabbit pAb
Catalog No.A5268
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 273-372 of human CXCR5 (NP_001707.1).
SequenceSPYHIVIFLDTLARLKAVDNTCKLNGSLPVAITMCEFLGLAHCCLNPMLYTFAGVKFRSDLSRLLTKLGCTGPASLCQLFPSWRRSSLSESENATSLTTF
Gene ID
Swiss Prot
SynonymsBLR1; CD185; MDR15
Calculated MW42kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesB cells, BT-474, Raji, Mouse liver
Cellular locationCell membrane, Multi-pass membrane protein
Customer validation

IHC(Mus musculus)

ABclonal:Western blot - CXCR5 Rabbit pAb (A5268)}

Western blot - CXCR5 Rabbit pAb (A5268)

Western blot analysis of extracts of various cell lines, using CXCR5 antibody (A5268) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)